A preclinical small animal imaging platform combining multi-angle photoacoustic and fluorescence projections into co-registered 3D maps

Authors: Weylan Thompson1, Anthony Yu2,3, Diego S. Dumani2,3,4, Jason Cook5, Mark A. Anastasio6, Stanislav Y. Emelianov2,3, Sergey A. Ermilov1

Affiliations

1 PhotoSound Technologies, Inc. (United States)
2 Georgia Institute of Technology (United States)
3 Emory Univ. School of Medicine (United States)
4 Univ. de Costa Rica (Costa Rica)
5 NanoHybrids, Inc. (United States)
6 Univ. of Illinois at Urbana-Champaign (United States)

Abstract

We present the results on development of the 3D imaging platform combining photoacoustic tomography and fluorescence (PAFT) for preclinical and biological research. This combined multimodal imaging instrument addresses known deficiencies in sensitivity, spatial resolution, and anatomical registration of the individual imaging components. Multiangle photoacoustic projections, excited by an OPO operating in the near-infrared window, of a live anesthetized animal are used to reconstruct large volumes (30 cm3) that show deep anatomical vasculature and blood-rich tissues with resolutions exceeding 150 μm. A sCMOS camera is used for simultaneous co-registered multi-angle optical imaging. The images of a fluorescent dual-contrast agent are then reconstructed into a 3D volume using a tomographic algorithm. A separate 532-nm low-energy pulsed laser excitation is used for skin topography and imaging of superficial vasculature. All three imaging channels can be combined to produce spatially accurate in vivo volumes showing an animal’s skin, deep anatomical structures, and distribution of photosensitive molecular contrast agents. PAFT’s photoacoustic sensitivity was assessed using contrast agents in a phantom study. We demonstrate biomedical imaging application of PAFT’s combined imaging modalities by observing biodistribution of a dual-contrast agent injected intravenously to in vivo preclinical murine models.

Reference

Journal: Proceedings Volume 11240, Photons Plus Ultrasound: Imaging and Sensing 2020; 112400L (2020)
Event: SPIE BiOS, 2020, San Francisco, California, United States