Date & Time: June 29, 2023 10:00 AM CT
Description
Overview:
The talk will focus on the synthesis, characterization and use of a novel contrast agent composed of indocyanine green dye for NIR-I photoacoustic imaging. The contrast agent can be easily tuned to different sizes without enclosure in nanocarriers, has strong optical absorption and PA signal at 895 nm, can be easily functionalized with different targeting molecules and can be imaged for 120 minutes in vivo. The presentation will explain details on the genesis of the idea for building a biocompatible contrast agent and give details on its easy synthesis protocols, touch upon a functionalization scheme for adding targeting molecules and demonstrate its use as a PA contrast in mice using the TriTom small animal imaging system.
In this webinar, we will present a synthesis method for clinically translatable ICG-JA whose mean size can be finely tuned from 200 nm to 1000 nm and that does not require encapsulation in a nanocarrier. The talk will also detail complete characterization of the agent and steps for functionalization with targeting peptides or antibodies. Additionally, the webinar will also provide details about the PA properties of the contrast in vitro in different conditions including whole blood, followed by details on the photoacoustic imaging in vivo using the TriTom system.
Learning Objectives:
- Audience will get details on the synthesis of a NIR contrast agent without the need of a nanocarrier.
- They can learn in detail about what characteristics a contrast agent should possess to qualify as a clinically translatable technology.
- Audience will also be familiar with methods to create a targeted contrast agent.
- Audience will also see the use of this contrast agent in imaging small animals such as mice and its compatibility with a system such as the TriTom.