Tag: fluorescence imaging

Probing the photophysical properties of fluorescent proteins using photoacoustic pump-probe spectroscopy and imaging

Author(s): F. Ghane Golmohamadi, A. Mehmood, H. T. Phan, F.-J. Schmitt, and J. Laufer ABSTRACT Pump-probe excitation of fluorophores has been shown to overcome the limitations of conventional multiwavelength imaging and linear unmixing approaches by providing fluorophore-specific contrast whilst eliminating the dominant background signal of endogenous chromophores. In this study, methods for generating pump-probe signals and images are […]