Tag: in vivo

CO-REGISTERED PHOTOACOUSTIC AND FLUORESCENT IMAGING OF A SWITCHABLE NANOPROBE BASED ON J-AGGREGATES OF INDOCYANINE GREEN

Authors: Diego S. Dumani, Hans-Peter Brecht, Vassili Ivanov, Ryan Deschner, Justin T. Harris, Kimberly A. Homan, Jason R. Cook, Stanislav Y. Emelianov, Sergey A. Ermilov ABSTRACT We introduce a preclinical imaging platform – a 3D photoacoustic/fluorescence tomography (PAFT) instrument augmented with an environmentally responsive dual-contrast biocompatible nanoprobe. The PAFT instrument was designed for simultaneous acquisition […]

A PRECLINICAL SMALL ANIMAL IMAGING PLATFORM COMBINING MULTI-ANGLE PHOTOACOUSTIC AND FLUORESCENCE PROJECTIONS INTO CO-REGISTERED 3D MAPS

Authors: Weylan Thompson, Anthony Yu, Diego S. Dumani, Jason Cook, Mark A. Anastasio, Stanislav Y. Emelianov, Sergey A. Ermilov ABSTRACT We present the results on development of the 3D imaging platform combining photoacoustic tomography and fluorescence (PAFT) for preclinical and biological research. This combined multimodal imaging instrument addresses known deficiencies in sensitivity, spatial resolution, and […]

PRECLINICAL SMALL ANIMAL IMAGING PLATFORM PROVIDING CO-REGISTERED 3D MAPS OF PHOTOACOUSTIC RESPONSE AND FLUORESCENCE

Authors: Diego S. Dumani1,2, Anthony Yu1,2, Weylan Thompson3, Hans-Peter Brecht3, Vassili Ivanov3, Mark A. Anastasio4, Jason Cook5, Sergey A. Ermilov3, Stanislav Y. Emelianov1,2 ABSTRACT We report on the development of a preclinical 3D imaging platform integrating photoacoustic tomography and fluorescence (PAFT). The proposed multimodal imaging concept addresses known deficiencies in sensitivity, anatomical registration, and spatial […]

DUAL-MODALITY X-RAY-INDUCED RADIATION ACOUSTIC AND ULTRASOUND IMAGING FOR REAL-TIME MONITORING OF RADIOTHERAPY

Authors: Wei Zhang,1 Ibrahim Oraiqat,2 Hao Lei,3 Paul L. Carson,1,4 Issam EI Naqa,2 and Xueding Wang1,4 ABSTRACT Objective. The goal is to increase the precision of radiation delivery during radiotherapy by tracking the movements of the tumor and other surrounding normal tissues due to respiratory and other body motions. Introduction. This work presents the recent advancement of X-ray-induced radiation acoustic imaging (xRAI) […]

GOLD NANOPARTICLES CONJUGATED WITH DNA APTAMER FOR PHOTOACOUSTIC DETECTION OF HUMAN MATRIX METALLOPROTEINASE-9

Authors: Jinhwan Kim, Anthony M Yu, Kelsey P. Kubelick, Stanislav Y. Emelianov Fig. 5. Spectroscopic analysis and PAT of the MMP sensor in an in vivo mouse model of breast cancer. (a) PA intensity from 680 nm to 970 nm of the MMP sensor in the tumor region and assorted controls – saline only and control sensor. Black dashed line […]

PHOTOACOUSTIC TOMOGRAPHY TO ASSESSACUTE VASOACTIVITY OF SYSTEMIC VASCULATURE

Author(s): Huda, Kristieª; Lawrence, Dylanª; Lindsey, Sarah; Bayer, Carolynª* ABSTRACT Vasoactivity is an important physiological indicator of cardiovascular health which is frequently measured using ex vivo vessels to determine functional mechanisms and evaluate pharmacological responses. Currently, there are no imaging methods available to assess vasoactivity in multiple vascular beds of living animals noninvasively. In this […]

PHOTOACOUSTIC IMAGING FOR IN VIVO QUANTIFICATION OF ALCOHOL-INDUCED STRUCTURAL AND FUNCTIONAL CHANGES IN CEREBRAL VASCULATURE IN HIGH ALCOHOL-PREFERRING MICE (HAP)

Authors: Augustine Meombe Mbolle, Hao Yang, Huabei Jiang* ABSTRACT Alcohol-induced structural and functional changes were studied in vivo by photoacoustic tomography (PAT) of the cerebrovascular system in selectively bred alcohol-preferring mice. High (HAP) and low (LAP) alcohol-preferring mice are replicate lines of mice selectively bred to prefer 10% (v/v) ethanol to water and water to […]